Regulatory
pLux/cI-OR

Part:BBa_K415031:Design

Designed by: Grant Robinson   Group: iGEM10_MIT   (2010-10-22)


Improved PLux/cI-OR Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This promoter was designed entirely as a result of issues with the R0065 promoter, which was essentially designed as a literal fusion of two promoters that happened to contain twin -10 transcription initiation sites. This resulted in a promoter that was not leaky without the presence of AHL, but constitutively on. This newly designed promoter should properly follow the logic detailed above.


Source

This is a hybrid promoter synthesized from a portion of the Vibrio Fischerii Lux operon and the cI/cro switch of Lambda bacteriophage.

References